Separate-Vial Reconstitution Spec BAC water added BPC-157 (10 mg vial) 2.0 mL TB-500 (10 mg vial) 2.0 mL Spec Final concentration BPC-157 (10 mg vial) 5.0 mg/mL TB-500 (10 mg vial) 5.0 mg/mL Spec 10 units (0.10 mL) delivers BPC-157 (10 mg vial) 500 mcg TB-500 (10 mg vial) 500 mcg Spec 20 units (0.20 mL) delivers BPC-157 (10 mg vial) 1.0 mg TB-500 (10 mg vial) 1.0 mg Spec Doses per vial (typical) BPC-157 (10 mg vial) ~2040 days TB-500 (10 mg vial) ~45 loading doses This separate-vial row keeps the same 10 mg-per-compound assumption as the 20 mg pre-blend, but the compounds are reconstituted and drawn independently
In brief, HUVECs were lysed in Co-IP buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, and 0.5% protease inhibitor cocktail on ice for 20 min
Immunohistochemistry and immunofluorescence Serial Sect
If any step breaks down (low stomach acid, lack of intrinsic factor, or gut inflammation), much of the B12 passes through unabsorbed
Your body already makes it