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The complexity of neurosteroid-mediated effects, for instance gating of GABA A R (109, 135139) and subunit-specific modulation of glycine and NMDARs (140), suggest the likelihood of multiple binding sites that contribute to potentiating and inhibitory effects ( A Rs also appears to involve modulation of subunit-containing receptors which play a role in tonic inhibition (141145)

By comparing reconstructed 3D volumes of each cluster, the particle stacks representing distinct states were identified and subsequently subjected into the non-uniform consensus refinement and the receptor-focused refinement

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