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The enhanced cocktails, CILy (CHIR+IDE1+Ly) and CIP (CHIR+IDE1+PD), significantly increased the expression of DE markers SOX17 and FOXA2, with CIP showing the highest efficiency, comparable to the traditional CA method
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10.1093/brain/awv082 392 ZhangC.YangY.LiangW.WangT.WangS.WangX.et al
If initial staining was insufficient, the sample was sectioned again to obtain a better visual field of cartilage structure
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