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Male SpragueDawley (SD) rats weighing 200 20 g were obtained from the Animal Experiment Center of the Fourth Military Medical University (Xian, China) and housed in a specific pathogen-free animal facility

refrigerate at 2-8C after reconstitution

2.2.4 Trigger day We monitored the ovarian follicle developmental progress using vaginal ultrasound scanning and estradiol levels during treatment

10.1016/j.biopha.2018.10.034 14 ClabaultH.CohenM.VaillancourtC.SandersonJ

Flow cytometry using the DCFH-DA probe indicated that the marked elevation in ROS levels caused by the OGD and erastin treatments was rescued by the GSH treatment (Fig