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To further confirm this observation, we transfected HepG2 cells with either scrambled siRNA or siRNA targeting GS overnight, followed by TNF challenge
It is likely that variations in experimental methods, biological systems, as well as the distinct metabolic characteristics of cells contributed to the variability in reported results 18,23
Sitagliptin was not mutagenic or clastogenic with or without metabolic activation in the Ames bacterial mutagenicity assay, a Chinese hamster ovary (CHO) chromosome aberration assay, an in vitro cytogenetics assay in CHO, an in vitro rat hepatocyte DNA alkaline elution assay, and an in vivo micronucleus assay
however, the precise effects of chronic glucagon receptor agonism on human GI physiology are not yet fully established
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