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To make the hEC surface charge more positive the glycocalyx of hECs was digested with neuraminidase enzyme (1 U/ml, 1-h pretreatment) or cultures were treated with cationic lipid 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH
NMR spectroscopy NMR experiments were performed using a Bruker 900 MHz NMR spectrometer equipped with a cryogenic probe (KBSI, Ochang) and a Bruker 600 MHz NMR spectrometer equipped with a cryogenic probe (GIST, Gwangju)
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