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The practical advantage of epitalon lies in its standardization and reproducibility
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To validate macrophage activation, mouse bone marrow-derived macrophages were extracted and incubated with cancer cells, that have been treated with the nanoparticles
The samples were rotated at room temperature for 3 hours before washing with the 2 nd wash buffer (50 mM TEAB, 150 mM NaCl, 0.1% NP-40) 3 times, 3 times with PBS and 3 times with water
PubMed PMID: 22074294
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