After this point, hepatocytes (liver cells) are unable to keep up
As expected, H 2 O 2 (0.2 mM) exposure disrupted cellular glutathione homeostasis in both HepG2 cells (a human hepatoma cell line) and NCTC1469 cells (a non-transformed mouse hepatocyte cell line), as demonstrated by an increase in the GSSG/GSH ratio over time (Fig
To show the outlines of acinar cells, we used Alexa Fluor 488 conjugated Wheat Germ Agglutinin (1:200) as counterstaining (W11261, Thermo Fisher, Waltham, MA, USA)
Methods ICU patient blood collection The prospective cohort study in adult critically ill patients was approved by the Ethical Committee of the Ghent University Hospital (Belgian registration number of the study: B670201213147), and conducted in accordance with the declaration of Helsinki and in compliance with the Good Clinical Practice Guidelines
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