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This pattern is reported across forums and is consistent with IGF-1's known effects on intracellular hydration and protein synthesis through PI3K/Akt/mTOR (Yoshida & Delafontaine, 2020)

First, the images were converted to gray-scale, then the threshold for fluorescence was set based on the positive and negative control images

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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IFNg

Plates were placed in a motorized stage microscope (EVOS Auto FL 2, Thermo Fisher Scientific, Waltham, MA) equipped with an on-stage incubator and maintained at 5% CO 2 and 37C