[PubMed abstract ( 17
Notably, the highest QE was observed at pH 6.5, which was selected as the optimal pH for further experiments
It was shown recently that treatment of erythrocytes with bacterial neuraminidase leads to desialylation of the glycocalyx and provokes cell surface PS exposure [216]
Flow cytometry and sorting For surface staining cells were incubated at 48 C, in the dark with fluorochrome-conjugated CD19, CD23, CD21-CD35 or CD35, IgD, IgM, CD1d, CD24, CD93, CD95, and GL-7 diluted 1:200 (refer to Supplementary Data 4 for details)
Injection equipment, such as needles and syringes, should always be sterile and for single use only