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Diabetes Care 25 (12), 21652171

Stress and Sleep Chronic stress and poor sleep increase cortisol levels, encouraging fat storage and reducing energy expenditure

After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample