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RNA yield and integrity were initially assessed from absorbance readings at 260 and 280 nm using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, Loughborough, UK), and confirmed using an Agilent Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent Technologies, Wokingham, UK) according to the manufacturers guidelines

When the data did not meet these assumptions, non-parametric tests were applied, namely the Kruskal-Wallis test followed by Dunns post hoc test

Manufacturers frequently incorporate cholesterol at a 7:3 molar ratio to increase membrane rigidity and prevent premature leakage of the active tripeptide during storage

However, especially under high stress from environmental influences, sports, or illness, the glutathione level can decrease

Study methods and models vary by institution and protocol