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In humans, TB4 is present in high concentrations in all tissue types except red blood cells, with highest levels occurring in platelets, white blood cells, plasma, and wound fluid.17 TB4 is an important actin-binding protein that helps actin perform various functions, such as muscular contraction and maintaining proper cell shape, movement, and division.18 19 These effects on cells are why some experts suggest supplemental TB4 can improve the repair and recovery of muscle and other soft tissues,20 21 promote wound healing,22 reduce inflammation, and more.23 Researchers also note that TB4 is critical for the repair and regeneration of eye, heart, skin, nerve, and brain tissue after injury.4 The derivative TB-500 is based on one of the active sites of TB4, specifically the peptide segment responsible for the compounds actin-binding and cell migration abilities.4 Since most of TB4s potential benefits are attributed to its actin-binding properties, it can be assumed that the same applies to TB-500

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To 1 ml of OD 600 40 culture, 10 l probe ( Metro-P1 , Metro-P2 or Metro-P3 , 100 stock in DMSO, final concentration 1 M) or 1% (v/v) DMSO were added and incubated at 37 C (140 rpm, microaerophilic conditions) for 2 h and subsequently collected by centrifugation (6,000 g , 10 min, 4 C)