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To analyze the gut microbiota composition, the mRNA expression of specific microbial genes (Table 2) in the fecal samples was quantified following the same qPCR protocol used for mouse liver tissue
Following incubation, the absorbance at 450 nm was measured for each well using a microplate reader
Bioscience, biotechnology, and biochemistry
The stock solution of trace elements contained the following in 3 M HCl: FeSO 4 7H 2 O 80, AlCl 3 6H 2 O 10, ZnSO 4 7H 2 O 2.0, CuCl 2 2H 2 O 1.0, NaMoO 4 2H 2 O 2.0, MnSO 4 nH 2 O 10, CoCl 2 4.0, and H 3 BO 4 0.5 g/L
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