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Although pro-oxidant profile was evident in both brain areas, differences in the magnitude of some alterations and in aconitase activity suggested that the functional consequences of oxidative stress may vary across brain regions

Phytotherapy research: PTR.Aug 14 2017

Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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When this teamwork flows smoothly, your body can handle normal chemical exposures without breaking a sweat