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Vitamin C Vitamin C (ascorbic acid) is a well-known antioxidant involved in collagen synthesis and oxidative defence

The nuclear pellet was washed and then lysed in buffer C (20 mM HEPES, pH 7.9, 200 mM NaCl, 1 mM EDTA, 5% v v 1 glycerol, 1 mM DTT, 1 mM Na 3 VO 4 , 1 mM PMSF, and 10 mg mL 1 aprotinin and leupeptin)

Regular follow-up is necessary Just giving the injection is not enough