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The WT or mutant GIPR was transiently transfected using Lipofectamine 2000 transfection reagent as previous described 25
This reaction is important because the H 2 O 2 might reduce the life expectancy of the erythrocytes for the increase in the speed of hemoglobin oxidation to metahemoglobin2 (Figure 1)
10.1016/j.redox.2017.11.001 Redox Biol
Additionally, it features structural modifications that significantly extend its half-life
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