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After 24 h, viability was assessed using the AquaBluer assay as previously described ( Determination of GSH Levels HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco), and maintained in a humidified atmosphere with 5% CO 2 at 37C

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Person two wonders why the medication does not seem to do anything