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Either too early or after the 8-hour mark
The mixture was then shaken vigorously at 15 C for 2 h at 250 rpm, followed by centrifugation at 12,000 g for 15 min
Pull-down assay and immunoblotting Interactions between exendin 939 and TRPV1 were examined via His-mediated pull-down assays using a modified protocol from the Pierce Pull-down PolyHis Protein:Protein Interaction Kit (Thermo Fisher Scientific)
Immunohistochemical detection of gamma-glutamyl transpeptidase in normal human tissue
Surgery reduces healthcare costs within 25 years by lowering medication use, hospitalizations, and disability [175]
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