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And thats all before downstream purification begins, which requires its own lengthy steps, including traditional chromatography
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Stefani A, Lozano AM, Peppe A, Stanzione P, Galati S, Tropepi D, et al
As a research reagent it is used to probe type 1 IGF receptor activation, downstream PI3K and MAPK signalling, IGF binding protein biology, and the specific question of how much native IGF-I behaviour reflects IGFBP sequestration rather than receptor pharmacology
The post describes the anterior and lateral, or outer, thigh as the safest zone, roughly one handspan below the hip bone and one handspan above the knee, where subcutaneous tissue runs about 8 to 15 mm deep in most adults
