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Then, 300 l of UPLC grade H 2 O were added (giving a final chloroform:methanol:water ratio of 2:1:3), vortexing was repeated and the sample was centrifuged at 16,000 g for 5 min

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This might explain the common x axis intercept in LineweaverBurk plots 11,17,18 , which resemble a non-competitive inhibition pattern with identical dissociation constants for the inhibitor and substrate 52

| BPC-157 is a synthetic peptide derived from a naturally occurring protein in gastric juice, primarily studied for its tissue-protective and regenerative effects