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ura K, Hionaka Y, Sakurai I (1990) Effect of carnitine on size limitation of experimental myocardial infarct - PubMed
Hearts were minced finely in isolation buffer (300 mM sucrose, 10 mM Hepes, 2 mM EGTA, pH 7.2) at 4C, in the presence of 0.5 M type I protease (bovine pancreas) to release mitochondria from the muscle fibers, and then washed in the same buffer in the presence of 1 mg/mL BSA
Human clinical trials conducted over extended observation periods reveal remarkable mortality benefits
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