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The cell suspension was sonicated using mechanical ultrasonicator (4 s intervals of 50 Hz repeated 10 times) followed by 3 cycles of freezing of cell lysate in liquid N 2 and thawing at room temperature
doi: 10.1038/s41574-019-0165-y 84 RyuVGarretsonJTLiuYVaughanCHBartnessTJ
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