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(6) Acetaldehyde has toxic effects such as damage to mitochondria due to alterations in the cell membrane, DNA damage, reduction of oxygen utilization, cell death due to decreases in enzyme activity of the proteins that degrade reactive oxygen species (ROS) such as glutathione s transferase (GST) and lipid peroxidation

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Heat at 95C for 5 minutes unless the antibody datasheet recommends another condition