Fish, Diets and Sampling In order to avoid the metabolic disturbance of estrogen during the sexual maturation of female fish, only male zebrafish (0.25 0.03 g) were bought from Chinese National Zebrafish Resource Center (Wuhan, China)
Following the recommended escalation schedule of at least 4 weeks per dose level minimizes discomfort
Structure Sequence: Glycyl-L-Histidyl-L-Lysine Copper (GHK-Cu) Molecular Formula: C14H24N6O4Cu Molecular Weight: 403.92 g/mol PubChem CID: 3081431 Citations for GHK-Cu Collagen Synthesis & Skin Rejuvenation Pickart, L., et al
Location The experiment was conducted at Paysand Center, property of Universidad Nacional de Colombia, located in a tropical lower montane wet forest region according to the Holdridge classification of life zones ( Espinal, 1977 )
General Notes A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay