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Unstable iron (mainly in the form of ferrous iron) can react with oxygen and produce ROS, such as hydroxyl radicals and hydrogen peroxide, which are related to lipid peroxidation and tissue damage induced through the Fenton reaction

Additionally, histological assessment involved Tyrosine Hydroxylase (TH) staining in the substantia nigra to evaluate the integrity and potential restoration of dopaminergic neurons, with evaluations performed at 1 month and 3 months post-lesion

It also happens within the mitochondria , which are the "powerhouses" of your cells that produce energy