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The protein bands were detected using enhanced chemiluminescence (ECL) reagent (Pierce ECL Western Blotting Substrate, Thermo Scientific) according to the manufacturers instructions
Specifically, the increased loss of RBCs leads to the increased formation of bilirubin, which further triggers eryptosis by enhancing both Ca 2+ influx, SMase activation, and ceramide production, thus creating a vicious cycle
Dreisler E, Kjer JJ
& van Kuilenburg, A
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