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The mix was incubated for 2 min and then 1 l of Thermoscript reverse transcriptase (200 U) (Invitrogen) was added and incubated for 50 min at 42 C
We generally recommend one injection per week
Hugs are awesome
Each trial continued until the mouse was unable to sustain running for more than 1015 s, despite gentle encouragement
Next, we assessed the impact of SLC25A39 on CRC cell migration using scratch wound healing and transwell chamber assays
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