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The digest was filtered through a 70-m mesh and centrifuged using a 28.7% Nycodenz gradient (Nycomed PharmaAS, Oslo, Norway)
Although the smaller number of differentially expressed proteins in the kidney compared with the renal artery limits broader conclusions, these findings indicate that chronic loss of VSMC GLP-1R signaling is accompanied by detectable basal proteomic alterations at both the vascular and organ level
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