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Robb EL, Hall AR, Prime TA, Eaton S, Szibor M, Viscomi C, et al
Advancements in Regenerative Therapies for Orthopedics: A Comprehensive Review of Platelet-Rich Plasma, Mesenchymal Stem Cells, Peptide Therapies, and Biomimetic Applications
I've also seen people spend money on gray-market versions that accomplished nothing
Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected
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