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An initial fungal cell density of OD 600 = 0.03 was inoculated into the 100 mL enriched chytrid growth medium in 250 mL flasks and cultured on a shaker incubator at 30 C, 150 rpm under continuous illumination (~ 10 mol m 2 s 1 )

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This approach significantly amplified anti-tumor efficacy while minimizing systemic toxicity, presenting a novel paradigm for precision cancer therapy [194]