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Further mechanism exploration revealed that lncRNA TALC induced TMZ resistance in GBM through trapping miR-20b-3p and activating c-Met
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Each step builds on the last, creating a cascade of improvement that addresses PCOS at its root rather than merely treating symptoms
A standard expression induction protocol was based on shifting log-phase cultures (A600 = ~0.6) from 37 C and adding IPTG (1 mM)
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