The infection in freshwater fish presents variation, including the species and size of fish, sources of areas, types of water, and seasons, which is determined by the interaction among environmental factors, liver fluke prevalence in definite hosts, distribution of first intermediate hosts, and biology and behavior of freshwater fish (104)
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Olzmann JA, Li L, Chin LS
Chang C-H, Tsai W-C, Hsu Y-H, Pang J-HS
If the precipitate does not dissolve, you may need to dilute it with fresh DMSO or re-prepare the stock solution