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cDNA was produced from mRNA using TaqMan reverse transcription reagents (Applied Biosystems, Foster City, CA, USA)

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However, in Alzheimer's and other neurodegenerative diseases the visual cortex has been found to be particularly resistant to change, and references measurements from that region would be worth obtaining at least on an exploratory basis to assess if it reduced inter subject variation in the measurement of metabolite changes in the disease affected volume

reported that intravenous GLP-1 administration attenuated the hypotensive response in healthy older individuals (90)