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Following purification, mRNA was fragmented into short fragments using a NEB Next Magnesium RNA Fragmentation Module (New England Biolabs, Ipswich, MA, USA) at 94C for 5-7 minutes
Discussion As one of the most complex biological processes that occur during human life, the wound repair process involves four steps that include inflammation around the site of injury, angiogenesis and the development of granulation tissue, repair of the connective tissue and epithelium, and ultimately remodeling that leads to a healed wound
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