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For quantitative real-time PCR (qPCR) assays, expression levels of rat 2A -adrenergic receptor ( Adra2a ) were detected using the QuantiTect SYBR Green PCR Kit (Qiagen) and SYBR Select Master Mix (Sigma-Aldrich) and quantified according to the 2 Ct method

The PCR products were gel-extracted, purified and cleaned (Macherey-Nagel, 740609) and 200 ng of each laccase mutant gene was transformed along with 100 ng of linearized vector into S

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Someone with dental amalgams might need different approaches than occupational exposure cases

And you have to do it gently