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Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark

Encyclopedia of Food and Health (Elsevier, 2016)

According to studies, glutathione lowers blood lactic acid levels, which in turn helps lower blood pressure

3) Incubate at 37C, 5% CO 2 for 4 hours, then harvest cells