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Alcohol-wipe your surface, wash up, glove if you can, and lay everything within reach

Some researchers prefer to add more water (3-5 mL) to create a more dilute solution that requires a larger, easier-to-measure draw

Therefore, M199 supplemented with 20 mM HEPES and 10% iFBS appeared to be suitable for incubation of NTS by vortex transformation and was used for the drug sensitivity assays