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Interestingly, concurrent same-day colonoscopy was identified as a protective factor, with a lower risk of food retention (OR 0.18, p -value = 0.003) in patients undergoing both EGD and colonoscopy [29]
protect from light by keeping the vial in its carton and never freeze the oil solution, as crystallisation or vial breakage can occur at sub-zero temperatures
2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added
doi: 10.1128/mBio.02706-20 156 AbedJEmgrdJEMZamirGFarojaMAlmogyGGrenovAet al
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