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At the end of its 2 year shelf life, when stored in the refrigerator, there are approximately 350 mg
True irritation requiring product discontinuation presents as persistent burning or stinging lasting beyond application period, significant redness spreading beyond application zone or lasting multiple hours, swelling of eyelids or periorbital area, increased sensitivity making area uncomfortable to touch, or itching suggesting allergic response
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Note also that coordination involves loss of protons from the ligand, so the observed mass of the complex is not simply the peptide mass plus the copper mass
Reconstitution is required
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