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Differing cytotoxicity and bioavailability of selenite, methylselenocysteine, selenomethionine, selenosugar 1 and trimethylselenonium ion and their underlying metabolic transformations in human cells

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The p Ka of the putative catalytic cysteine is lower in GRXC2 than in ROXY9 To investigate the potential reactivity of Cys A of ROXY9 and GRXC2 as a nucleophile under physiological p H , we compared the apparent p K a values of the cysteines of strep-MBP-ROXY9 and strep-MBP-GRXC2

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Results showed that metformin could increase p-AMPK protein level in a dose-dependent manner, whereas liraglutide did not have such effect as metformin (Supplemental Fig