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Ahmad and M
As a positive control for apoptosis, tamoxifen (50 M) was included ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells
Analytical Quality Manufactured to exceed 99% purity and independently tested using HPLC and LC-MS analytical methods to support identity confirmation, purity evaluation, and batch-to-batch consistency
The disulfide form of cysteine, cystine, is an especially reactive amino acid substrate of this cycle
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