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Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted with TRIzol reagent (Ambion, Austin, TX, USA) and reverse-transcribed using PrimeScript RT kit (TaKaRa, Shiga, Japan)

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Prolonged sun exposure: Spending long periods in direct sunlight may trigger hyperpigmentation, as it promotes increased production of melanin (which is why we tan)

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