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By splitting an aptamer that binds to ATP and labeling each fragment with differently colored fluorophores, they were able to develop an assay that reports binding only when the two fluorophores are in proximity to each other, and thus eliminates background from non-specific binding events
Specifically, it was shown to potently stimulate insulin release in response to glucose, and to restrict glycemic excursion, beyond that of the GLP-1 albumin fusion composed of WT albumin, likely due to a higher concentration in plasma
The assay demonstrates high sensitivity, as evidenced by its low detection limits (LOD) and quantification (LOQ) of 3 U/L and 1 U/L, respectively
Switching to a different GLP-1 medication does not change the underlying mechanism
In most cases the hair loss on Zepbound is a telogen effluvium, that is, a diffuse shedding across the whole scalp
