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2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions
Journal of Parenteral and Enteral Nutrition, 36 (2), 147154
Ensure that the needle is bevel up and that you fully insert it to reach the muscle
Diabetes Care 37 , 30163024 (2014)
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