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Subsequently, the cells were lysed in a hypertonic buffer comprising 20 mM Tris-HCl (pH 7.4), 300 mM NaCl, 10 mM MgCl 2 , 0.5% sodium deoxycholate, 1% Triton X-100, 1 mM DTT, 100 g/ml CHX, and 300 U/ml of RNase inhibitor

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This intensive initial treatment rapidly replenishes depleted B12 stores and addresses acute symptoms

These fulfil the deficiencies in the body caused by multiple reasons such as pregnancy, breast feeding, lack of sleep , anxiety ,loss of appetite ,unbalanced food intake, chronic disease, blood deficiency anaemia ,unknown causes, skin problems etc