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"Nitrous oxide has multiple deleterious effects on cobalamin metabolism and causes decreases in activities of both mammalian cobalamin-dependent enzymes in rats"
Circular dichroism spectroscopy, which we did to understand the secondary structure of our best candidate bovine IF, which indicated a predominantly helical secondary structure consistent with native folding, while computational docking predicted bovine and human IF to have optimal geometric complementarity and stabilising energies with B12, in agreement with our experimental binding data
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The benzyl alcohol acts as a bacteriostatic agent
Understanding which combinations make mechanistic sense and which should be avoided is important for safe and effective protocols
