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Protein expression was analysed and detected using the Western Lightning Plus-ECL, Enhanced Chemiluminescence Substrate (PerkinElmer, NEL105001EA) and G:BOX ChemiXX6 gel doc system with GeneSys image software (Syngene)
It was the first peptide of its kind, described by Cyril Bowers in 1984
Esr1 -deficient mice ( B6N(Cg)-Esr1 tm4.2Ksk /J ) 53 were purchased from Jackson Laboratories and bred from heterozygous male and female mice
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