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To further explain the relationship between pyruvate-boosted cysteine and pyruvate-depressed GSH, the expression of genes encoding the conversion from cysteine to GSH and from GSH to glycine was measured and the activity of GPX and GR was detected in the presence of different concentrations of pyruvate
Seungpyo Hong and Ryan Pearson for helpful discussions of experiments for this manuscript
As a result of this strong orbital mixing and electron transfer during the CASSCF(14,13) geometric optimization, the Co-C and Co-O bond lengths in cyanocobalamin, methylcobalamin, and aquacobalamin increase steadily until they entirely break at the end of the optimization process
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