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While KYN itself is not neuroactive, it is a branching point for two major metabolic routes: one leading to the synthesis of kynurenic acid (KYNA), which occurs in astrocytes, and another leading to the synthesis of picolinic acid (PA) and quinolinic acid (QA), occurring in microglia, with QA serving as a precursor for nicotinamide adenine dinucleotide (NAD) [25, 26]

Ray SD, Lam TS, Rotollo JA, Phadke S, Patel C, Dontabhaktuni A, Mohammad S, Lee H, Strika S, Dobrogowska A, Bruculeri C, Chou A, Patel S, Patel R, Manolas T, Stohs S

Liochev SI, Fridovich I

Adding less liquid concentrates the solution, which decreases the draw volume

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